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KMID : 0545120120220091224
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Journal of Microbiology and Biotechnology
2012 Volume.22 No. 9 p.1224 ~ p.1229
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Enzymatic Synthesis of Puerarin Glucosides Using Leuconostoc Dextransucrase
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Ko Jin-A
Ryu Young-Bae
Park Tae-Soon
Jeong Hyung-Jae
Kim Jang-Hoon
Park Su-Jin
Kim Joong-Su
Kim Do-Man
Kim Young-Min
Lee Woo-Song
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Abstract
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Puerarin (P), an isoflavone derived from kudzu roots, has strong biological activities, but its bioavailability is often limited by its low water solubility. To increase its solubility, P was glucosylated by three dextransucrases from Leuconostoc or Streptococcus species. Leuconostoc lactis EG001 dextransucrase exhibited the highest productivity of puerarin glucosides (P-Gs) among the three tested enzymes, and it primarily produced two P-Gs with a 53% yield. Their structures were identified as α-D-glucosyl- (1→6)-P (P-G) by using LC-MS or 1H- or 13C-NMR spectroscopies and α-D-isomaltosyl-(1→6)-P (P-IG2) by using specific enzymatic hydrolysis, and their solubilities were 15- and 202-fold higher than that of P, respectively. P-G and P-IG2 are easily applicable in the food and pharmaceutical industries as alternative functional materials.
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KEYWORD
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puerarin, dextransucrase, Leuconostoc lactis, water solubility, transglucosylation
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